Journal: Nucleic acids research

Article Title: Sequestration of ribosomal subunits as inactive 80S by targeting eIF6 limits mitotic exit and cancer progression.

doi: 10.1093/nar/gkae1272

Figure Lengend Snippet: Figure 9. Ov ere xpression of eIF6 observ ed primarily in high-grade in v asiv e bladder and breast cancers. ( A and B ) Western blot probed for eIF6 in human bladder cancer cell lines and healthy (normal) bladder epithelial cells. β-Tubulin used as loading control. Blots shown in panel (A) were quantitated and eIF6 le v els w ere normaliz ed to loading control and plotted (panel B) as FC o v er eIF6 le v els in normal bladder epithelial cells. Values indicate standard error of the mean from four independent experiments and significant differences for T24 ( P = 0.0065), UMUC3 ( P = 0.0132) and HT1197 ( P = 0.0017) determined by an unpaired t wo-t ailed t -test. ( C ) Images represent eIF6 expression in patient-derived benign and high-grade tumors by immunohistochemistry using anti-eIF6 antibody. Enlarged inset shows the presence of eIF6 in nucleoli and cytoplasm in high-grade cancers. ( D ) Images shown in panel (C) were quantitated and eIF6 expression in patient-matched benign tissues relative to high-grade cancers (six patients) were plotted. Significant differences were determined using an unpaired t wo-t ailed t -test ( P = 0.0063). ( E ) Plot shows eIF6 expression in unmatched benign tissues, high-grade and low-grade bladder cancers derived from patients. ( F and G ) Western blot represents high levels of eIF6 in high-grade invasive human triple negative breast cancer cell line. β-Tubulin used as loading control. Blots shown in panel (F) were quantitated and eIF6 levels were normalized to loading control and plotted (G) as FC o v er eIF6 le v els in normal (healthy) HME-1 cells. Values indicate standard error of the mean from four independent experiments and significant differences for MDA-MB-231 ( P = 0.001) determined by an unpaired t wo-t ailed t -test.

Article Snippet: The human bladder cancer cell lines HT1376, HT1197, UM-UC-3 and TCCSUP were maintained in EMEM media with 10% FBS and Penn / Strep, T-24 cell lines were maintained in McCoy’s 5A medium with 10% FBS and Penn / Strep and primary bladder epithelial (A / T / N): normal human (BdEC) cells were maintained in bladder epithelial cell basal medium (ATCC) supplemented with bladder epithelial g c i g b D W C [ a p 1 fl d t ( m a p c w m ( b l o β S 0 p B S C C e d s i S B m B s a ( S B a p a m t w s i i H 7 w f v D ow nloaded from https://academ ic.oup.com /nar/advance-article/doi/10.1093/nar/gkae1272/7933602 by guest on 30 D ecem ber 2024 rowth factors.

Techniques: Western Blot, Control, Expressing, Derivative Assay, Immunohistochemistry

Journal: Cell genomics

Article Title: A cis -regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation

doi: 10.1016/j.xgen.2022.100191

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Primary Human Bladder Epithelial Cells (A/T/N) (Bladder) were obtained from ATCC (PCS-420–010) and grown in Bladder Epithelial Cell Basal Medium ((ATCC PCS-420–032) supplemented with Bladder Epithelial Cell Growth Kit (ATCC PCS-420–042).

Techniques: Recombinant, SYBR Green Assay, Staining, HiChIP, Software

Journal: Cancer Science

Article Title: Differential expression of S100A2 and S100A4 in lung adenocarcinomas: Clinicopathological significance, relationship to p53 and identification of their target genes

doi: 10.1111/j.1349-7006.2005.00121.x

Figure Lengend Snippet: mRNA levels for the S100 family in small airway epithelial cells (SAEC) and seven lung adenocarcinoma cell lines

Article Snippet: Cell lines and medium Normal human bronchial epithelial cell (NHBE) lines and SAEC (small airway epithelial cells) were purchased from BioWhittaker (Walkerville, MD, USA) and cultured in medium provided by the manufacturer.

Techniques:

Journal: Cancer Science

Article Title: Differential expression of S100A2 and S100A4 in lung adenocarcinomas: Clinicopathological significance, relationship to p53 and identification of their target genes

doi: 10.1111/j.1349-7006.2005.00121.x

Figure Lengend Snippet: Expression analyses of S100A2 and S100A4 in bronchial epithelial cells (NHBE and SAEC) and nine lung adenocarcinoma cell lines. Levels of S100A2 (a) and S100A4 (b) mRNA were determined by real‐time reverse transcription–polymerase chain reaction analyses. Protein levels of S100A2 (c) and S100A4 (d) were examined by western blot analysis. The lower panels in (c) and (d) represent β‐actin expression (arrows) serving as an internal control. Lanes: 1, NHBE; 2, SAEC; 3, LC‐2/ad; 4, ABC‐1; 5, A549; 6, H460; 7, HLC1; 8, H1299; 9, PC3; 10, VMRC‐LCD; 11, RERF‐LC‐KJ.

Article Snippet: Cell lines and medium Normal human bronchial epithelial cell (NHBE) lines and SAEC (small airway epithelial cells) were purchased from BioWhittaker (Walkerville, MD, USA) and cultured in medium provided by the manufacturer.

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Western Blot, Control